dNTPs

Deoxyribose nucleoside triphospates (dNTPs) is a mixture of the four monomeric units, dATP, dTTP, dCTP and dGTP that are the substrates for new DNA synthesis. Denaturation step in PCR results in single stranded DNA molecules and in the extension step, DNA polymerase adds these free dNTPs from the reaction mixture to the template DNA in the 5’-to-3’ direction in a stepwise and drives the polymerization reaction forward.